FACIAL SOFT TISSUE THICKNESS (FSTT) AND ITS SEXUAL DIMORPHISM IN A MALAYSIAN SAMPLE: A CT-BASED APPROACH


Date Published : 11 December 2025

Contributors

Faiz Fauad

Author

Aspalilah Alias

Author

Choy Ker Woon

Author

Helmi Mohd Hadi

Author

Keywords

Sex Facial soft tissue thickness CT variations Jantina ketebalan tisu lembut wajah variasi

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Copyright (c) 2025 Faiz Fauad; Aspalilah Alias; Choy Ker Woon; Helmi Mohd Hadi;

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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Abstract

Facial soft tissue thickness (FSTT) is a crucial element in forensic identification and facial reconstruction. Exploring its variations according to different sexes provides valuable information that is needed for an accurate identification. This study aimed to investigate FSTT variations between males and females of adult Malaysian populations. A total of 413 head and neck computed tomography (CT) scans (208 males, 205 females) of patients aged 18–65 years were selected.  Four mid-sagittal landmarks; nasion, mid-philtrum, subnasale, and upper lip were involved in the measurement, using 3D-Slicer software. The effect of sex on FSTT was analysed using multivariate analysis of variance (MANOVA). MANOVA showed a statistically significant difference in FSTT between sexes (Wilks’ Lambda = 0.020, p < 0.001). All four landmarks demonstrated significant variations between males and females (all p < 0.001). Males have greater FSTT compared to females at all landmarks. In conclusion, there were significant FSTT variations between the sexes of adult Malaysian population. This population-specific data could be applied as forensic reference that tailored to Malaysian population.

Ketebalan tisu lembut wajah (Facial Soft Tissue Thickness, FSTT) merupakan elemen penting dalam proses identifikasi forensik dan pembinaan semula wajah. Penelitian terhadap variasinya mengikut jantina memberikan maklumat kritikal yang diperlukan bagi memastikan ketepatan identifikasi. Kajian ini bertujuan menyiasat perbezaan FSTT antara lelaki dan perempuan dalam kalangan populasi dewasa di Malaysia. Sebanyak 413 imbasan tomografi berkomputer (CT) kepala dan leher (208 lelaki, 205 perempuan) melibatkan pesakit berumur 18–65 tahun telah dipilih. Empat titik penanda mid-sagital—nasion, mid-philtrum, subnasale dan bibir atas—digunakan dalam pengukuran melalui perisian 3D-Slicer. Kesan jantina terhadap FSTT dianalisis menggunakan analisis varians multivariat (MANOVA). Keputusan MANOVA menunjukkan perbezaan statistik yang signifikan dalam FSTT antara jantina (Wilks’ Lambda = 0.020, p < 0.001). Keempat-empat titik penanda menunjukkan variasi yang signifikan antara lelaki dan perempuan (semua p < 0.001). Lelaki mempunyai nilai FSTT yang lebih besar berbanding perempuan pada semua titik penanda. Kesimpulannya, terdapat variasi FSTT yang signifikan antara jantina bagi populasi dewasa Malaysia. Data khusus populasi ini boleh digunakan sebagai rujukan forensik yang disesuaikan dengan populasi Malaysia.

References

Agbolade, O., Nazri, A., Yaakob, R., Ghani, A. A., & Cheah, Y. K. (2020). Morphometric approach to 3D soft-tissue craniofacial analysis and classification of ethnicity, sex, and age. PLoS ONE, 15(4), 1–24. https://doi.org/10.1371/journal.pone.0228402
Alazzawi, S., Omar, R., Rahmat, K., & Alli, K. (2012). Radiological analysis of the ethmoid roof in the Malaysian population. Auris Nasus Larynx, 39(4), 393–396. https://doi.org/10.1016/j.anl.2011.10.002
Alhumadi, A., Al-Khafaji, T. J., Alyassiri, A. M. H., & Alhamadi, W. W. (2022). Gender differences in lower facial soft tissue thickness among different skeletal patterns, based on soft tissue cephalometric analysis. Journal of Orthodontic Science, 11(1).
Ayoub, F., Saadeh, M., Rouhana, G., & Haddad, R. (2019). Midsagittal facial soft tissue thickness norms in an adult Mediterranean population. Forensic Science International, 294, 217.e1–217.e7.
Berar, M., Desvignes, M., Bailly, G., & Payan, Y. (2005). 3D statistical facial reconstruction. In Proceedings of the 4th International Symposium on Image and Signal Processing and Analysis (pp. 365–370).
Brobbey, A., Wiebe, S., Nettel-Aguirre, A., Josephson, C. B., Williamson, T., Lix, L. M., et al. (2022). Repeated measures discriminant analysis using multivariate generalized estimation equations. Statistical Methods in Medical Research, 31(4), 646–657.
Bulut, O., Sipahioglu, S., & Hekimoglu, B. (2014). Facial soft tissue thickness database for craniofacial reconstruction in the Turkish adult population. Forensic Science International, 242, 44–61.
Chan, C. Y., Subramaniam, S., Mohamed, N., Ima-Nirwana, S., Muhammad, N., Fairus, A., et al. (2020). Determinants of bone health status in a multi-ethnic population in Klang Valley, Malaysia. International Journal of Environmental Research and Public Health, 17(2), 1–16.
Chen, H. S., Hsiao, S. Y., & Lee, K. T. (2021). Analysis of facial skeletal morphology: Nasal bone, maxilla, and mandible. BioMed Research International, 2021.
Cheng, C. H., Chen, L. R., & Chen, K. H. (2022). Osteoporosis due to hormone imbalance: An overview of the effects of estrogen deficiency and glucocorticoid overuse on bone turnover. International Journal of Molecular Sciences.
Chin, K. Y., Soelaiman, I. N., Mohamed, I. N., Ahmad, F., Ramli, E. S. M., Aminuddin, A., et al. (2013). Sex hormones in Malay and Chinese men in Malaysia: Are there age and race differences? Clinics, 68(2), 159–165.
Chung, J. H., Chen, H. T., Hsu, W. Y., Huang, G. S., & Shaw, K. P. (2015). A CT-scan database for the facial soft tissue thickness of Taiwan adults. Forensic Science International, 253, 132.e1–132.e11.
Domaracki, M., & Stephan, C. N. (2006). Facial soft tissue thicknesses in Australian adult cadavers. Journal of Forensic Sciences, 5–10.
Eggerstedt, M., Rhee, J., Buranosky, M., Batra, P. S., Tajudeen, B. A., Smith, R. M., et al. (2020). Nasal skin and soft tissue thickness variation among differing races and ethnicities: An objective radiographic analysis. Facial Plastic Surgery & Aesthetic Medicine, 22(3), 188–194.
Emmanuelle, N. E., Marie-Cécile, V., Florence, T., Jean-François, A., Françoise, L., Coralie, F., et al. (2021). Critical role of estrogens on bone homeostasis in both male and female: From physiology to medical implications. International Journal of Molecular Sciences.
Fedorov, A., Beichel, R., Kalpathy-Cramer, J., Finet, J., Fillion-Robin, J. C., Pujol, S., et al. (2012). 3D Slicer as an image computing platform for the quantitative imaging network. Magnetic Resonance Imaging, 30(9), 1323–1341.
De Greef, S., Claes, P., Vandermeulen, D., Mollemans, W., Suetens, P., & Willems, G. (2006). Large-scale in-vivo Caucasian facial soft tissue thickness database for craniofacial reconstruction. Forensic Science International, 159(1).
Gupta, S., Gupta, V., Vij, H., Vij, R., & Tyagi, N. (2015). Forensic facial reconstruction: The final frontier. Journal of Clinical and Diagnostic Research, 26–28.
Hamid, S., & Abuaffan, A. H. (2016). Facial soft tissue thickness in a sample of Sudanese adults with different occlusions. Forensic Science International, 266, 209–214.
Ichikawa-Seki, M., Hayashi, K., Tashiro, M., & Khadijah, S. (2022). Dispersal direction of Malaysian Fasciola gigantica from neighboring Southeast Asian countries inferred using mitochondrial DNA analysis. Infection, Genetics and Evolution, 105.
Iqbal, S. P., Ramadas, A., Fatt, Q. K., Shin, H. L., Onn, W. Y., & Kadir, K. A. (2020). Relationship of sociodemographic and lifestyle factors and diet habits with metabolic syndrome (MetS) among three ethnic groups of the Malaysian population. PLoS ONE, 15(3), 1–16. https://doi.org/10.1371/journal.pone.0224054
Ismail, N. A., Abu Bakar, S. N., Abdullah, N., Shafie, M. S., & Mohd Nor, F. (2019). Stature estimation in the South-East Asian population: A systematic review. Malaysian Journal of Pathology, 41(2), 83–89.
Khosla, S., Oursler, M. J., & Monroe, D. G. (2012). Estrogen and the skeleton. Trends in Endocrinology and Metabolism, 576–581.
Kumar, T. S., & Vijai, A. (2012). 3D reconstruction of face from 2D CT scan images. Procedia Engineering, 970–977.
Lefevre, C. E., Lewis, G. J., Perrett, D. I., & Penke, L. (2013). Telling facial metrics: Facial width is associated with testosterone levels in men. Evolution and Human Behavior, 34(4), 273–279.
Leth, P. M. (2007). The use of CT scanning in forensic autopsy. Forensic Science, Medicine, and Pathology, 3(1), 65–69.
Lundy, J. K. (1998). Forensic anthropology: What bones can tell us. Laboratory Medicine, 29(7), 423–427.
Marianetti, T. M., Boccieri, A., & Pascali, M. (2016). Reshaping of the anterior nasal spine: An important step in rhinoplasty. Plastic and Reconstructive Surgery Global Open, 4(9).
McDuff, D., Kodra, E., El Kaliouby, R., & LaFrance, M. (2017). A large-scale analysis of sex differences in facial expressions. PLoS ONE, 12(4).
Miller, E. (2018). Forensic anthropology. In Multidisciplinary Medico-Legal Death Investigation: Role of Consultants.
Mohamad, N. V., Soelaiman, I. N., & Chin, K. Y. (2016). A concise review of testosterone and bone health. Clinical Interventions in Aging, 1317–1324.
Moore, M. K. (2012). Sex estimation and assessment. In Research Methods in Human Skeletal Biology (pp. 91–116). Elsevier.
Musilová, B., Dupej, J., Brůžek, J., Bejdová, Š., & Velemínská, J. (2019). Sex- and ancestry-related differences between two Central European populations determined using exocranial meshes. Forensic Science International, 297, 364–369.
Narla, R. R., & Ott, S. M. (2018). Bones and the sex hormones. Kidney International, 239–242.
Nilendu, D., & Johnson, A. (2023). Role of soft-tissue thickness on the reproducibility in forensic facial approximation: A comparative case study. Forensic Science International: Reports, 7.
Pasca, P., & Ulasan, K. (2014). Decomposition process and post mortem changes: Review. Sains Malaysiana.
Perović, T., & Blažej, Z. (2018). Male and female characteristics of facial soft tissue thickness in different orthodontic malocclusions evaluated by cephalometric radiography. Medical Science Monitor, 24, 3415–3424.
Randolph-Quinney, P. S., Black, S. M., & Mallett, X. (2011). Forensic anthropology. In A. Jamieson & A. Moenssens (Eds.), Wiley Encyclopedia of Forensic Science (pp. 1–27). John Wiley & Sons Ltd.
Rozendaal, A. S., Scott, S., Peckmann, T. R., & Meek, S. (2020). Estimating sex from the seven cervical vertebrae: An analysis of two European skeletal populations. Forensic Science International, 306, 110072. https://doi.org/10.1016/j.forsciint.2019.110072
Saadeh, M., Fayyad-Kazan, H., Haddad, R., & Ayoub, F. (2020). Facial soft tissue thickness differences among different vertical facial patterns. Forensic Science International, 317.
Schumann, N. P., Bongers, K., Scholle, H. C., & Guntinas-Lichius, O. (2021). Atlas of voluntary facial muscle activation: Visualization of surface electromyographic activities of facial muscles during mimic exercises. PLoS ONE, 16(7).
Schutte, H., Muradin, M. S. M., Bielevelt, F., Seubring, K., Bleys, R. L. A. W., & Rosenberg, A. J. W. P. (2023). The average facial expressions: A range of motion analysis for different sex and age groups. Plastic and Reconstructive Surgery Global Open, 11(1), e4762.
Shamaei-Tousi, A., Veneziano, A., & Landi, F. (2022). Relation between nasal septum deviation and facial asymmetry: An ontogenetic analysis from infants to children using geometric morphometrics. Applied Sciences, 12(22).
Spradley, M. K., & Jantz, R. L. (2011). Sex estimation in forensic anthropology: Skull versus postcranial elements. Journal of Forensic Sciences, 56(2), 289–296.
Stavrianos, C., Stavrianou, I., Zouloumis, L., & Mastages, D. (2007). An introduction to facial reconstruction. Balkan Journal of Stomatology, 11, 76–83.
Stephan, C. N., & Preisler, R. (2018). In vivo facial soft tissue thicknesses of adult Australians. Forensic Science International, 282, 220.e1–220.e12.
Tan, Y. R., Tan, E. H., Jawahir, S., Mohd Hanafiah, A. N., & Mohd Yunos, M. H. (2021). Demographic and socioeconomic inequalities in oral healthcare utilisation in Malaysia: Evidence from a national survey. BMC Oral Health, 21(1).

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Faiz, F., Aspalilah , A. ., Choy Ker, C. K., & Helmi , H. . (2025). FACIAL SOFT TISSUE THICKNESS (FSTT) AND ITS SEXUAL DIMORPHISM IN A MALAYSIAN SAMPLE: A CT-BASED APPROACH. International Conference on Syariah, Law and Science (CFORSJ I-CONF) - CFORSJ Procedia, 3(1), 95-102. https://alnadwah.usim.edu.my/cforsjprocedia/paper/view/162